Extracting signal from noise: kinetic mechanisms from a Michaelis-Menten-like expression for enzymatic fluctuations
نویسندگان
چکیده
منابع مشابه
Extracting signal from noise: kinetic mechanisms from a Michaelis-Menten-like expression for enzymatic fluctuations.
Enzyme-catalyzed reactions are naturally stochastic, and precision measurements of these fluctuations, made possible by single-molecule methods, promise to provide fundamentally new constraints on the possible mechanisms underlying these reactions. We review some aspects of statistical kinetics: a new field with the goal of extracting mechanistic information from statistical measures of fluctua...
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Most biological processes are controlled by complex systems of enzymatic chemical reactions. Although the majority of enzymatic networks have very elaborate structures, there are many experimental observations indicating that some turnover rates still follow a simple Michaelis-Menten relation with a hyperbolic dependence on a substrate concentration. The original Michaelis-Menten mechanism has ...
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A biodegradable polyester resin was polymerized from N-benzyloxycarbonylL -glutamic acid and ethylene glycol. Rhizopus delemar lipase was used as a biocatalyst for the rupture of ester bonds during the hydrolysis studies. Depolymerization was observed to followa Michaelis–Menten mechanism, with the maximumrate of monomer formation dP/dtmax = 1.12 x 10-8 mol/s and the rate constant Km x 2.03 x 1...
متن کاملA Comparison of Estimates of Michaelis-menten Kinetic Constants from Various Linear Transformations.
The parameters which characterize this equation, and which must ordinarily be estimated from the observed data, are ‘v,,,, the maximum initial velocity which is theoretically attained when the enzyme has been “saturated” by an infinite concentration of substrate, and K,, the Michaelis constant which is numerically equal to the concentration of substrate for half-maximal initial velocity. Since ...
متن کاملA Comparison of Estimates of Michaelis-Menten Kinetic Constants from Various Linear Transformations
The parameters which characterize this equation, and which must ordinarily be estimated from the observed data, are ‘v,,,, the maximum initial velocity which is theoretically attained when the enzyme has been “saturated” by an infinite concentration of substrate, and K,, the Michaelis constant which is numerically equal to the concentration of substrate for half-maximal initial velocity. Since ...
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ژورنال
عنوان ژورنال: FEBS Journal
سال: 2013
ISSN: 1742-464X
DOI: 10.1111/febs.12545